Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | activates | Raphanus sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucose | very weak inhibition | Raphanus sativus | |
SO42- | - |
Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
sinigrin | in absence of ascorbate | Raphanus sativus | |
0.25 | - |
sinigrin | in presence of 0.5 mM ascorbate | Raphanus sativus | |
14 | - |
p-nitrophenyl-beta-D-glucopyranoside | in absence of ascorbate | Raphanus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
1 * 61000 + 1 * 62000, SDS-PAGE | Raphanus sativus |
62000 | - |
1 * 61000 + 1 * 62000, SDS-PAGE | Raphanus sativus |
120000 | - |
gel filtration | Raphanus sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Raphanus sativus | the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Raphanus sativus | - |
seedling | - |
Raphanus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
164 | - |
- |
Raphanus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates | Raphanus sativus | ? | - |
? | |
p-nitrophenyl beta-D-glucopyranoside + H2O | - |
Raphanus sativus | ? | - |
? | |
sinigrin + H2O | - |
Raphanus sativus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 61000 + 1 * 62000, SDS-PAGE | Raphanus sativus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.11 | - |
sinigrin | in absence of ascorbate | Raphanus sativus | |
287 | - |
sinigrin | in presence of 0.5 mM ascorbate | Raphanus sativus |