Cloned (Comment) | Organism |
---|---|
orf0643, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces sp. JHA26 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncation of the PA14 domain of the enzyme (N-terminal 179 amino acid residues), ORF0643 Galf-ase lacking the PA14 domain exhibits about half activity compared with full-length ORF0643 | Streptomyces sp. JHA26 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
4-nitrophenyl beta-D-galactofuranoside | pH 4.5, 37°C, recombinant enzyme | Streptomyces sp. JHA26 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. JHA26 | A0A1Q2U3E4 | isolated from soil in Kagawa University, Japan | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces sp. JHA26 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces sp. JHA26 | 4-nitrophenol + D-galactofuranose | - |
? | |
additional information | the enzyme is active with a galactofuranose-containing oligosaccharide termed GM | Streptomyces sp. JHA26 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-galactofuranosidase | - |
Streptomyces sp. JHA26 |
galactofuranose-specific beta-D-galactofuranosidase | - |
Streptomyces sp. JHA26 |
Galf-ase | - |
Streptomyces sp. JHA26 |
Galf-specific Galf-ase | - |
Streptomyces sp. JHA26 |
Galf-specific hydrolase | - |
Streptomyces sp. JHA26 |
ORF0643 | locus name | Streptomyces sp. JHA26 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces sp. JHA26 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant detagged enzyme, pH 5.5, 10 min, stable up to, unstable above | Streptomyces sp. JHA26 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
recombinant enzyme | Streptomyces sp. JHA26 |
General Information | Comment | Organism |
---|---|---|
additional information | ORF0643 contains PA14, glycosyl hydro 2 N, glycosyl hydro and glycosyl hydro 2C domains | Streptomyces sp. JHA26 |