KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.145 | - |
4-O-Methyl-glucuronosyl-xylotriose | - |
Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
1 * 107000, SDS-PAGE | Thermoascus aurantiacus |
118000 | - |
gel filtration | Thermoascus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermoascus aurantiacus | the three enzymes, xylanase, alpha-D-glucuronidase, and beta-glucosidase, together are responsible for complete degradation of larchwood xylan to xylose and 4-O-methyl-alpha-D-glucuronic acid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoascus aurantiacus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Thermoascus aurantiacus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.98 | - |
- |
Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-alpha-D-glucuronosyl-xyloheptaose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-alpha-D-glucuronate + xyloheptaose | - |
? | |
4-O-methyl-glucuronosyl-xylobiose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-glucuronate + xylobiose | - |
? | |
4-O-methyl-glucuronosyl-xylohexaose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-glucuronate + xylohexaose | - |
? | |
4-O-methyl-glucuronosyl-xylopentaose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-glucuronate + xylopentaose | - |
? | |
4-O-methyl-glucuronosyl-xylose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-glucuronate + xylose | - |
? | |
4-O-methyl-glucuronosyl-xylotetraose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-glucuronate + xylotetraose | - |
? | |
4-O-methyl-glucuronosyl-xylotriose + H2O | - |
Thermoascus aurantiacus | 4-O-methyl-glucuronate + xylotriose | - |
? | |
additional information | the three enzymes, xylanase, alpha-D-glucuronidase, and beta-glucosidase, together are responsible for complete degradation of larchwood xylan to xylose and 4-O-methyl-alpha-D-glucuronic acid | Thermoascus aurantiacus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 107000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | 40°C: about 35% of maximal activity, 75°C: about 30% of maximal activity | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
1 mg/ml of substrate protects against heat inactivation | Thermoascus aurantiacus |
50 | - |
in absence of substrate, 6 h, 15% loss of activity | Thermoascus aurantiacus |
60 | - |
in absence of substrate, t1/2: 192 min | Thermoascus aurantiacus |
60 | - |
in absence of substrate, 6 h, 58% loss of activity | Thermoascus aurantiacus |
70 | - |
in absence of substrate, t1/2: 28 min | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5.8 | about 45% of maximal activity at pH 3 and at pH 5.8 | Thermoascus aurantiacus |