Activating Compound | Comment | Organism | Structure |
---|---|---|---|
xylanase | when the enzyme is used in combination with xylanase, a large stimulation in activity occurs | uncultured bacterium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysE cells | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ addition results in a slight activity decrease (86.3%) | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the presence of manganese produces higher activity (117.4%) | uncultured bacterium | |
additional information | enzyme activity is not dramatically impacted by Co2+, Cu2+, Fe2+, Li+, Mg2+, and Zn2+ at 1 mM | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84500 | - |
x * 84500, calculated from amino acid sequence | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | uncultured bacterium | - |
4-O-methyl-alpha-D-glucuronic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | I2E7L0 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | - |
uncultured bacterium | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84500, calculated from amino acid sequence | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucuronidase | - |
uncultured bacterium |
DEG75-AG | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | after 2 h at 40 and 50°C, the enzyme retains most of the initial activity. At 60 °C, half of the activity is lost at 30 min, and no activity remains after 1 h | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | there is a dramatic decrease in enzyme activity at pH 6.0 and pH 9.0, and there is little activity at pH 4.0 and pH 5.0 | uncultured bacterium |