Application | Comment | Organism |
---|---|---|
additional information | development of an activity assay with commercially available reagents that can be used to search DNA libraries for alpha-glucuronidase genes in a highthroughput, solid phase activity screen | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SOLR cells carrying pBS expression vectors encoding the alpha-glucuronidase gene | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldouronic acid + H2O | Geobacillus stearothermophilus | - |
? | - |
? | |
aldouronic acid + H2O | Geobacillus stearothermophilus T6 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus T6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldobiouronic acid + H2O | - |
Geobacillus stearothermophilus | 2 D-xylose + 4-O-methyl-D-glucuronic acid | - |
? | |
aldobiouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 2 D-xylose + 4-O-methyl-D-glucuronic acid | - |
? | |
aldopentaouronic acid + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
aldopentaouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
aldotetraouronic acid + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
aldotetraouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
aldotriouronic acid + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
aldotriouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
aldouronic acid + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
aldouronic acid + H2O | - |
Geobacillus stearothermophilus T6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucuronidase | - |
Geobacillus stearothermophilus |