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Literature summary for 3.2.1.130 extracted from
- A single tryptophan residue of endomannosidase is crucial for Golgi localization and in vivo activity (2007), Cell. Mol. Life Sci., 64, 1881-1889.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of endomannosidase from CHO-K1 cells, amplicon subcloned into the Hind III/Xho I sites of the expression vector pcDNA6/myc-His, in frame with the C-terminal myc- and His-tag. Myc-tagged CHO endomannosidase expressed in CHO Lec23 cells. Generation of rat/CHO endomannosidase hybrid constructs | Cricetulus griseus |
| myc-tagged rat endomannosidase expressed in CHO Lec23 cells, generation of rat/CHO endomannosidase hybrid constructs | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C188A | enzymatic activity can not be fully rescued with a back-engineered Cys188Ala CHO cell endomannosidase | Cricetulus griseus |
| C188S | enzymatic activity can not be fully rescued with a back-engineered Cys188Ser CHO cell endomannosidase | Cricetulus griseus |
| C188W | enzyme activity can be reversed in cells with a back-engineered Cys188Trp CHO cell endomannosidase, in particular N-glycans of alpha1-antitrypsin become fully processed | Cricetulus griseus |
| W188C | molecular defect in CHO cells, is functionally important since it alone results in endoplasmic reticulum mislocalization of endomannosidase and causes greatly reduced in vivo activity | Cricetulus griseus |
| W188C | processing of the oligosaccharides of alpha1-antitrypsin is reduced, greatly diminished enzyme activity | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Cricetulus griseus | 5783 | - |
| Golgi apparatus | - |
Cricetulus griseus | 5794 | - |
| Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
ER-retained CHO cell endomannosidase, immunoprecipitation/Western blot analysis | Cricetulus griseus |
| 53220 | - |
sequence analysis | Cricetulus griseus |
| 58000 | - |
Golgi-localized rat endomannosidase species, immunoprecipitation/Western blot analysis | Rattus norvegicus |
| 61000 | - |
Golgi-localized rat endomannosidase species, immunoprecipitation/Western blot analysis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
| Rattus norvegicus | Q5GF25 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| CHO-K1 cell | no activity due to molecular defect in endomannosidase, CHO cells have a W188C substitution, Trp188 is critical for Golgi localization and in vivo activity of endomannosidase | Cricetulus griseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| human alpha1-antitrypsin + H2O | - |
Cricetulus griseus | ? | - |
? |  |
| human alpha1-antitrypsin + H2O | - |
Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endomannosidase | - |
Cricetulus griseus |
| endomannosidase | - |
Rattus norvegicus |
| Golgi-endomannosidase | - |
Cricetulus griseus |
| Golgi-endomannosidase | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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