Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Glucosyl alpha1-3(1-deoxy)mannojirimycin | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Rattus norvegicus | 139 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
? * 56000 + ? * 60000, 8-10 subunits, SDS-PAGE | Rattus norvegicus |
60000 | - |
? * 56000 + ? * 60000, 8-10 subunits, SDS-PAGE | Rattus norvegicus |
560000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcMan9GlcNAc + H2O | Rattus norvegicus | processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? | |
GlcMan9GlcNAc + H2O | Rattus norvegicus Cd | processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
CD-strain | - |
Rattus norvegicus Cd | - |
CD-strain | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 10.855 units/mg, 1 unit is the amount of enzyme that catalyzes the release of 1000 dpm of glucosyl-alpha-3Man in 1 h | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, in intact Golgi membranes, 10% loss of activity within 6 months | Rattus norvegicus |
-80°C, purified enzyme, at 0.03 mg protein/ml pH 6.8, 50% loss of activity within 6 months, increased stability at 0.5 mg protein/ml, bovine serum albumin stabilizes | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcMan9GlcNAc + H2O | - |
Rattus norvegicus | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? | |
GlcMan9GlcNAc + H2O | processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units | Rattus norvegicus | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? | |
GlcMan9GlcNAc + H2O | - |
Rattus norvegicus Cd | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? | |
GlcMan9GlcNAc + H2O | processing enzyme, accomplishes deglucosylation of glycoproteins with N-linked carbohydrate units | Rattus norvegicus Cd | Man8GlcNAc + glucosyl alpha1,3-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | ? * 56000 + ? * 60000, 8-10 subunits, SDS-PAGE | Rattus norvegicus |