Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-butanol | 20 mM, activates due to transglycosylation activity with over 80% of p-nitrophenyl beta-D-glucopyranoside being converted to 1-butyl beta-D-glucopyranoside in the presence of Cbg1 and 100 mM 1-butanol | Agrobacterium tumefaciens | |
1-Heptanol | 20 mM, activates | Agrobacterium tumefaciens | |
1-Hexanol | 20 mM, activates | Agrobacterium tumefaciens | |
1-Octanol | 20 mM, activates | Agrobacterium tumefaciens | |
1-Pentanol | 20 mM, activates | Agrobacterium tumefaciens | |
1-propanol | 20 mM, activates, highest rate of consumption of p-nitrophenyl beta-D-glucopyranoside in the presence of 0.6 M 1-propanol | Agrobacterium tumefaciens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
deoxynojirimycin | strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
glucuronolactone | strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
isopropyl 1-thio-beta-D-glucopyranoside | competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate | Agrobacterium tumefaciens | |
additional information | not inhibited by 24 mM xylose | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Agrobacterium tumefaciens | |
0.005 | - |
p-nitrophenyl beta-D-xylopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.012 | - |
p-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.021 | - |
arbutin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.023 | - |
coniferin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.045 | - |
Salicin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.06 | - |
o-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.08 | - |
p-nitrophenyl beta-D-fucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.27 | - |
p-nitrophenyl alpha-L-arabinofuranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
4 | - |
p-nitrophenyl beta-D-galactopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88289 | - |
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation | Agrobacterium tumefaciens |
98000 | - |
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferin + H2O | Agrobacterium tumefaciens | natural substrate | D-glucose + coniferol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Cbg1 | Agrobacterium tumefaciens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
coniferin + H2O = D-glucose + coniferol | mechanism | Agrobacterium tumefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39 | 41 | pH 6.5, 30°C | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arbutin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
coniferin + H2O | natural substrate | Agrobacterium tumefaciens | D-glucose + coniferol | - |
? | |
coniferin + H2O | 20000fold preference for the natural substrate coniferin over cellobiose | Agrobacterium tumefaciens | D-glucose + coniferol | - |
? | |
additional information | enzyme has a high transglycosylation activity in the presence of alcohols, transglycosylation mechanism | Agrobacterium tumefaciens | ? | - |
? | |
o-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | beta-D-glucose + o-nitrophenol | - |
? | |
p-nitrophenyl alpha-L-arabinofuranoside + H2O | with moderate efficiency | Agrobacterium tumefaciens | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
p-nitrophenyl beta-cellobioside + H2O | releases only terminal glucose from the substrate | Agrobacterium tumefaciens | ? | - |
? | |
p-nitrophenyl beta-D-fucopyranoside + H2O | with moderate efficiency | Agrobacterium tumefaciens | beta-D-fucose + p-nitrophenol | - |
? | |
p-nitrophenyl beta-D-galactopyranoside + H2O | with low efficiency | Agrobacterium tumefaciens | beta-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | beta-D-glucose + p-nitrophenol | - |
? | |
p-nitrophenyl beta-D-xylopyranoside + H2O | 73% of the efficiency with p-nitrophenyl beta-D-glucopyranoside | Agrobacterium tumefaciens | beta-D-xylose + p-nitrophenol | - |
? | |
salicin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, about, SDS-PAGE, x * 88289, sequence calculation | Agrobacterium tumefaciens |
Synonyms | Comment | Organism |
---|---|---|
Cbg1 | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Agrobacterium tumefaciens | |
3.15 | - |
Salicin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
3.2 | - |
p-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
22.1 | - |
p-nitrophenyl beta-D-fucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
28.9 | - |
p-nitrophenyl beta-D-xylopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
46.6 | - |
p-nitrophenyl beta-D-galactopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
86.7 | - |
Salicin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
95.4 | - |
p-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
106 | - |
coniferin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
118 | - |
p-nitrophenyl alpha-L-arabinofuranoside | pH 6.5, 30°C | Agrobacterium tumefaciens | |
155 | - |
o-nitrophenyl beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Agrobacterium tumefaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00078 | - |
deoxynojirimycin | pH 6.5, 30°C | Agrobacterium tumefaciens | |
0.0039 | - |
glucuronolactone | pH 6.5, 30°C | Agrobacterium tumefaciens | |
2.9 | - |
D-glucose | pH 6.5, 30°C | Agrobacterium tumefaciens | |
5.6 | - |
isopropyl 1-thio-beta-D-glucopyranoside | pH 6.5, 30°C | Agrobacterium tumefaciens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agrobacterium tumefaciens | isoelectric focusing, pH gradient 4-7 | - |
4.5 |