Any feedback?
Literature summary for 3.2.1.114 extracted from
- Characterization and subcellular localization of human neutral class II alpha-mannosidase cytosolic enzymes/free oligosaccharides/glycosidehydrolase family 38/M2C1/N-glycosylation (2007), Glycobiology, 17, 1084-1093.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect with EDTA | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| preparation of expression construct containing 6 x His and tobacco etch virus protease recognition sites in the N-terminus of NAM and integrated into the Pichia pastoris chromosome under inducible alcohol oxygenase promoter. CHO-K1 cells transfected with pNAM-EGFP | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | completely inhibits | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | granularly distributed | Homo sapiens | 5829 | - |
| additional information | absent from the endoplasmic reticulum, lysosomes, and autophagosomes | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Homo sapiens | |
| Fe2+ | is more activated by Fe2+ than Co2+ | Homo sapiens | |
| Mn2+ | - |
Homo sapiens | |
| additional information | no effect with Zn2+, Ca2+, and Mg2+ | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112000 | - |
4 * 112000, SDS-PAGE | Homo sapiens |
| 468000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation of recombinant enzyme followed by two cobalt chelating chromatography steps | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Man)9GlcNAc + H2O | hydrolysis to (Man)5GlcNAc in the presence of Fe2+, Co2+, and Mn2+. Without activating cations the main reaction product is (Man)8GlcNAc | Homo sapiens | (Man)5GlcNAc + D-mannose | - |
? | |
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Homo sapiens | 4-nitrophenol + alpha-D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 112000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAM | - |
Homo sapiens |
| neutral alpha-mannosidase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal pH is between 6.25 and 6.75 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.25 | 8 | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
