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Literature summary for 3.2.1.113 extracted from

  • Ziegler, F.; Trimble, R.
    Glycoprotein biosynthesis in yeast Purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase (1991), Glycobiology, 1, 605-612 .
    View publication on PubMed
Search for more literature for 3.2.1.113

General Stability

General Stability Organism
trypsin digestion of intact, osmotically supported membranes has no effect on the subsequent release of active enzyme by CHAPS, while digestion in the presence of CHAPS with trypsin at only 0.01 mg/ml inactivates more than 80% of the enzyme during the 1 h incubation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
EGTA complete inhibition at 5 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 Man9GlcNAc2-[thyroglobulin] at pH 6.5, temperature not specified in the publication Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Saccharomyces cerevisiae 5783
-
microsome
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.01 mM Ca2+ stimulates the enzyme activity to 77%, while 0.1 mM Ca2+ restores nearly 90% of the original activity Saccharomyces cerevisiae
additional information not stimulated by Mg2+ or Mn2+ Saccharomyces cerevisiae
Zn2+ 0.1 mM Zn2+ stimulates the enzyme activity by 30% Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man9GlcNAc2 + H2O Saccharomyces cerevisiae
-
 Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and TSK 3000 matrix gel filtration Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
25°C, in 1 mM CHAPS at pH 6.5-6.7, several days, about 50% loss of activity Saccharomyces cerevisiae
4°C, in 1 mM CHAPS at pH 6.5-6.7, several weeks, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 from oligosaccharide-lipid + H2O good substrate Saccharomyces cerevisiae ?
-
 ?
lipid-linked Man7GlcNAc2 (isomer 7A1,3B1,3) + H2O 10% activity compared to Man9GlcNAc2 Saccharomyces cerevisiae ?
-
 ?
Man9GlcNAc2 + H2O
-
 Saccharomyces cerevisiae Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
-
 ?
Man9GlcNAc2-[thyroglobulin] + H2O good substrate Saccharomyces cerevisiae Man8GlcNAc2-[thyroglobulin] + D-mannose
-
 ?
additional information the enzyme does not hydrolyze invertase Man9GlcNAc, Manlalpha2ManlalphaOCH3 or 4-nitrophenyl alpha-D-mannopyranoside Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 43700 + 1 * 22300, calculated from amino acid sequence Saccharomyces cerevisiae
heterodimer 1 * 44000 + 1 * 23000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Man9 processing alpha-mannosidase
-
 Saccharomyces cerevisiae
Man9-alpha-mannosidase
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 6.8 50% of the enzyme activity remains at pH 6.0 and 6.8, and activity falls off rapidly above and below these values Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae isoelectric focusing
-
 5.3