Cloned (Comment) | Organism |
---|---|
into vector pPIC9k-His6 and expressed in Pichia pastoris GS115 strain under the control of AOX1 promoter | Lipomyces starkeyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM completely inhibits enzyme activity | Lipomyces starkeyi | |
Cu2+ | 1 mM completely inhibits enzyme activity | Lipomyces starkeyi | |
Fe3+ | 1 mM completely inhibits enzyme activity | Lipomyces starkeyi | |
SDS | 1 mM completely inhibits enzyme activity | Lipomyces starkeyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM stimulates 2fold | Lipomyces starkeyi | |
Co2+ | - |
Lipomyces starkeyi | |
K+ | - |
Lipomyces starkeyi | |
Mg2+ | 1 mM enhances enzyme activity by 145% | Lipomyces starkeyi | |
Mn2+ | - |
Lipomyces starkeyi | |
Na+ | - |
Lipomyces starkeyi | |
Ni2+ | - |
Lipomyces starkeyi | |
Zn2+ | - |
Lipomyces starkeyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
enzyme after deglycosylation, SDS-PAGE and Western blot analysis | Lipomyces starkeyi |
70000 | - |
SDS-PAGE and Western blot analysis | Lipomyces starkeyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lipomyces starkeyi | - |
- |
- |
Lipomyces starkeyi 1390 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
17.56fold by cation-exchange chromatography, 18.18fold followed by Ni-affinity chromatography | Lipomyces starkeyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.36 | - |
- |
Lipomyces starkeyi |
182 | - |
enzyme purified 17.56fold | Lipomyces starkeyi |
188.3 | - |
enzyme purified 18.18fold | Lipomyces starkeyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Lipomyces starkeyi | ? | - |
? | |
dextran + H2O | - |
Lipomyces starkeyi 1390 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, purified enzyme, SDS-PAGE and Western blot analysis | Lipomyces starkeyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lipomyces starkeyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Lipomyces starkeyi |