Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type pancreatic enzyme, in complex with inhibitors acarbose, isoacarbose, or acarviosine-glucose, hanging drop vapour diffusion method, 0.005 ml of protein solution containing 16.3 mg/ml protein is mixed with 0.005 ml of reservoir solution containing 60% 2-methyl-2,4-pentanediol and 100 mM cacodylate, pH 7.5, 1 month, X-ray diffraction structure determination and analysis at 1.9-2.2 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | binding mechanism, acarbose rearrangement mechanism implied by the kinetic and structural analysis, and potential pathways of rearrangement, enzyme-inhibitor complex crystal structure analysis | Homo sapiens | |
acarviosine-glucose | binding mechanism, resistance to rearrangement, enzyme-inhibitor complex crystal structure analysis | Homo sapiens | |
isoacarbose | binding mechanism, resistance to rearrangement, enzyme-inhibitor complex crystal structure analysis, binding highly perturbs catalytic residue D300 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04746 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | the active site structure involves the catalytic residues D197, E233, and D300, reaction mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-4-nitrophenyl alpha-D-maltotrioside + H2O | - |
Homo sapiens | ? | - |
? | |
alpha-maltotriosyl fluoride + H2O | - |
Homo sapiens | maltotriose + fluoride | - |
? | |
maltosyl fluoride + H2O | - |
Homo sapiens | maltose + fluoride | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.98 | - |
2-chloro-4-nitrophenyl alpha-D-maltotrioside | pH 7.0, 30°C | Homo sapiens | |
150 | - |
maltosyl fluoride | pH 7.0, 30°C | Homo sapiens | |
285 | - |
alpha-maltotriosyl fluoride | pH 7.0, 30°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |