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Literature summary for 3.1.6.21 extracted from
- Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase (2014), Protein Sci., 23, 1442-1450 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 1.8M (NH4)2SO4, 2% (w/v) PEG400, and 0.1M HEPES (pH 7.4) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D184N/H185A | inactive | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Escherichia coli |  |
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | - |
Escherichia coli | |
| Ni2+ | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Escherichia coli | |
| Zn2+ | contains zinc | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P32717 | - |
- |
| Escherichia coli DH5alpha | P32717 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SDS + H2O | - |
Escherichia coli | 1-dodecanol + sulfate + Na+ | - |
? | |
| SDS + H2O | - |
Escherichia coli DH5alpha | 1-dodecanol + sulfate + Na+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 70000, SDS-PAGE | Escherichia coli |
| homodimer | 2 * 73000, calculated from amino acid sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| type III alkylsulfatase | - |
Escherichia coli |
| yjcS | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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