Application | Comment | Organism |
---|---|---|
medicine | X-linked ichthyosis is ascribed to the non-functional ES resulting from point and/or deletion mutations in the ES gene | Homo sapiens |
pharmacology | enzyme inhibitors can be used as anti-tumor agents | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
full-length, active enzyme form, X-ray analysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00959 | - |
dehydroepiandrosterone sulfate | pH 8, 37°C | Homo sapiens | |
0.07275 | - |
Estrone sulfate | pH 8, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | membrane-bound | Homo sapiens | 5783 | - |
membrane | bound to the membrane of the endoplasmic reticulum | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states | Homo sapiens |
65000 | - |
x * 65000, SDS-PAGE | Homo sapiens |
250000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-linked glycosylation at two of the possible four sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
1.556 | - |
pH 8, 37°C, hydrolysis of dehydroepiandrosterone sulfate | Homo sapiens |
Storage Stability | Organism |
---|---|
room temperature, crystalline state of enzyme, more than 30 days, less than 33% loss of specific activity | Homo sapiens |
room temperature, n-octyl-beta-D-glucopyranoside, several weeks, nearly stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroepiandrosterone sulfate + H2O | - |
Homo sapiens | dehydroepiandrosterone + sulfate | - |
? | |
estrone sulfate + H2O | - |
Homo sapiens | estrone + sulfate | - |
? | |
additional information | ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Homo sapiens |
More | all oligomeric states between a monomer and a tetramer are possible, varying aggregation properties of enzyme under various buffer, pH, detergent and ionic strength conditions, gel filtration suggests ES to be predominantly a homotetramer, native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DHEAS | - |
Homo sapiens |
ES | - |
Homo sapiens |
estrone sulfatase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.48 | - |
dehydroepiandrosterone sulfate | pH 8, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |