Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.45 extracted from

  • He, X.; Pierce, O.; Haselhorst, T.; von Itzstein, M.; Kolarich, D.; Packer, N.H.; Gloster, T.M.; Vocadlo, D.J.; Qian, Y.; Brooks, D.; Kermode, A.R.
    Characterization and downstream mannose phosphorylation of human recombinant alpha-L-iduronidase produced in Arabidopsis complex glycan-deficient (cgl) seeds (2013), Plant Biotechnol. J., 11, 1034-1043.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.45

Application

Application Comment Organism
biotechnology the uncovering enzyme is generated as soluble enzyme and so can be used to conduct the in vitro phosphorylation of purified recombinant lysosomal enzymes Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of the enzyme in soluble form for conducting the in vitro phosphorylation of purified recombinant lysosomal enzymes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-[6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannosyl]-[alpha-L-iduronidase]-L-asparagine + H2O Homo sapiens
-
 N-acetyl-D-glucosamine + N-[6-phospho-alpha-D-mannosyl]-[alpha-L-iduronidase]-L-asparagine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the human uncovering enzyme is synthesized as a proenzyme that is activated by the endoprotease furin in the trans-Golgi network Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation soluble enzyme Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannosyl]-[alpha-L-iduronidase]-L-asparagine + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + N-[6-phospho-alpha-D-mannosyl]-[alpha-L-iduronidase]-L-asparagine
-
 ?
N-[6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannosyl]-[alpha-L-iduronidase]-L-asparagine + H2O the enzyme is also active on alpha-L-iduronidase that is recombinantly expressed in transgenic Arabidopsis thaliana seeds Homo sapiens N-acetyl-D-glucosamine + N-[6-phospho-alpha-D-mannosyl]-[alpha-L-iduronidase]-L-asparagine
-
 ?

Synonyms

Synonyms Comment Organism
GlcNAc-1-phosphodiester alpha-N-acetylglucosaminidase
-
 Homo sapiens
uncovering enzyme
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function most lysosomal enzymes including alpha-L-iduronidase require a mannose 6-phosphate tag for efficient uptake and lysosomal delivery in human cells. In mammalian cells, the mannose 6-phosphate tag is generated by the sequential action of two enzymes: UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase, also known as GlcNAc-1-phosphotransferase, and N-acetylglucosamine 1-phosphodiester alpha-N-acetylglucosaminidase, i.e. the uncovering enzyme Homo sapiens