Cloned (Comment) | Organism |
---|---|
expression in mouse L-cells, expression of UCE containing green fluorescent protein in place of the luminal domain in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H510stop | mutant of the cytosolic tail disrupting the NPF endocytosis motif | Homo sapiens |
Y488A | mutant of the cytosolic tail disrupting the tyrosine-based endocytosis motif | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | resides in the trans-Golgi network and cycles between the network and plasma membrane, type I membrane-spanning glycoprotein with a single 27-residue transmembrane domain and a 41-residue cytoplasmic tail | Homo sapiens | 139 | - |
plasma membrane | resides in the trans-Golgi network and cycles between the network and plasma membrane | Homo sapiens | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, sialylated UCE, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O | Homo sapiens | catalyzes a crucial step in lysosomal biogenesis, removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal hydrolases | N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | UCE is core glycosylated as it enters the endoplasmic reticulum, traverses the Golgi apparatus where the oligosaccahide is processed to a complex-type structure | Homo sapiens |
sialoprotein | addition of sialic acid in the trans-Golgi network | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O | removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal hydrolases | Homo sapiens | N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose | - |
? | |
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O | catalyzes a crucial step in lysosomal biogenesis, removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal hydrolases | Homo sapiens | N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, sialylated UCE, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
2-acetamido-2-deoxy-alpha-D-glucose 1-phosphodiester acetamidodeoxyglucohydrolase | - |
Homo sapiens |
alpha-N-acetyl-D-glucosamine-1-phosphodiester N-acetylglucosaminidase | - |
Homo sapiens |
alpha-N-acetylglucosaminyl phosphodiesterase | - |
Homo sapiens |
lysosomal alpha-N-acetylglucosaminidase | - |
Homo sapiens |
UCE | uncovering enzyme | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |