Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.4 extracted from

  • Dippe, M.; Mrestani-Klaus, C.; Schierhorn, A.; Ulbrich-Hofmann, R.
    Phospholipase D-catalyzed synthesis of new phospholipids with polar head groups (2008), Chem. Phys. Lipids, 152, 71-77.
    View publication on PubMed
Search for more literature for 3.1.4.4

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Streptomyces sp.
Ca2+
-
 Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
-
-
Streptomyces sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.006
-
 transphosphatidylation reaction, substrate: 2-[bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol, specific activity of hydrolysis reaction: 0.029 micromol/min/mg Brassica oleracea
2.28
-
 transphosphatidylation reaction, substrate: tris(hydroxymethyl)-aminomethane, specific activity of hydrolysis reaction: 0.90 micromol/min/mg Brassica oleracea
4.08
-
 transphosphatidylation reaction, substrate: triethanolamine, specific activity of hydrolysis reaction: 5.68 micromol/min/mg Brassica oleracea
6.18
-
 transphosphatidylation reaction, substrate: diethanolamine, specific activity of hydrolysis reaction: 0.64 micromol/min/mg Brassica oleracea
7.53
-
 transphosphatidylation reaction, substrate: serinol, specific activity of hydrolysis reaction: 0.83 micromol/min/mg Brassica oleracea
9.19
-
 transphosphatidylation reaction, substrate: ethanolamine, specific activity of hydrolysis reaction: 0.65 micromol/min/mg Brassica oleracea
38.5
-
 transphosphatidylation reaction, substrate: 2-[bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol, specific activity of hydrolysis reaction: 22 micromol/min/mg Streptomyces sp.
124
-
 transphosphatidylation reaction, substrate: tris(hydroxymethyl)-aminomethane, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
223.5
-
 transphosphatidylation reaction, substrate: ethanolamine, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
380.3
-
 transphosphatidylation reaction, substrate: diethanolamine, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
410.1
-
 transphosphatidylation reaction, substrate: triethanolamine, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
489.7
-
 transphosphatidylation reaction, substrate: serinol, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dioleoyl-sn-glycerophosphocholine + 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol with BisTris as acceptor alcohol two regioisomeric forms of phosphatidyl-BisTris are obtained, product yield: 19.6% (regioisomer I), 3,1% (regioisomer II), purity of product: 98% (regioisomer I), 97% (regioisomer I) Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-2-[bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + diethanolamine product yield: 44.1%, purity of product: 97.0% Brassica oleracea choline + 1,2-dioleoyl-sn-glycerophospho-diethanolamine
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + diethanolamine product yield: 47.2%, purity of product: 81.5% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-diethanolamine
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + H2O
-
 Streptomyces sp. choline + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + serinol product yield: 39.5%, purity of product: 99.8% Brassica oleracea choline + 1,2-dioleoyl-sn-glycerophospho-serinol
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + serinol product yield: 45.8%, purity of product: 68% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-serinol
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + triethanolamine product yield: 22.6%, purity of product: 99.5% Brassica oleracea choline + 1,2-dioleoyl-sn-glycerophospho-triethanolamine
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + triethanolamine product yield: 33.7%, purity of product: 65.4% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-triethanolamine
-
 ?
1,2-dioleoyl-sn-glycerophosphocholine + tris(hydroxymethyl)-aminomethane product yield: 31.6%, purity of product: 95% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-tris(hydroxymethyl)-aminomethane
-
 ?
2 1,2-dioleoyl-sn-glycerophosphocholine + diethanolamine
-
 Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-diethanolamine
-
 ?
2 1,2-dioleoyl-sn-glycerophosphocholine + serinol
-
 Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-serinol
-
 ?
2 1,2-dioleoyl-sn-glycerophosphocholine + triethanolamine
-
 Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-triethanolamine
-
 ?
2 1,2-dioleoyl-sn-glycerophosphocholine + tris(hydroxymethyl)-aminomethane
-
 Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-tris(hydroxymethyl)-aminomethane
-
 ?

Synonyms

Synonyms Comment Organism
phospholipase D
-
 Streptomyces sp.
phospholipase D
-
 Brassica oleracea
PLDcab
-
 Brassica oleracea
PLDStr
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces sp.
30
-
 assay at Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 assay at Streptomyces sp.
5.6
-
 assay at Brassica oleracea