Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylinositol 4,5-bisphosphate | required | Arabidopsis thaliana | |
Triton X-100 | greatly stimulating | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression of variant PLDgamma2a in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | complete loss of activity and prohibition of any stimulation by phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Arabidopsis thaliana | |
Ca2+ | required, highest activity in presence of micromolar concentrations | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95600 | - |
x * 95600, calculated | Arabidopsis thaliana |
96000 | - |
x * 96000, calculated | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9T051 | isoform PLDgamma2 | - |
Arabidopsis thaliana | Q9T053 | isoform PLDgamma1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | presence of phosphatidylinositol 4,5-bisphosphate and phosphatidylethanol is required | Arabidopsis thaliana | 1,2-diacylglycerophosphate + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95600, calculated | Arabidopsis thaliana |
? | x * 96000, calculated | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | calculated | - |
8.3 |