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Literature summary for 3.1.4.39 extracted from
- The heterotrimeric G protein subunits Galphaq and Gbeta1 have lysophospholipase D activity (2011), Biochem. J., 440, 241-250.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| G protein | G protein exhibits lysoPLD activity, lysoPLD activity is highly associated with heterotrimeric G protein | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | heterotrimeric G protein subunits Galphaq and Gbeta1 have lysophospholipase D activity, choline production from lysoPAF by the purified FLAG-tagged Galphaq, overview. K52A, T186A, D205A, G48A and Q209L mutant forms of Galphaq show a significant reduction of lysoPLD activity, whereas G48V does not. The decrease of lysoPLD activity of G48A and Q209L is low | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from brain nuclear fractions by anion exchange chromatography, gel filtration, and again anion exchange chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | heterotrimeric G protein subunits Galphaq and Gbeta1 have lysophospholipase D activity, choline production from lysoPAF by the purified FLAG-tagged Galphaq, overview. K52A, T186A, D205A, G48A and Q209L mutant forms of Galphaq show a significant reduction of lysoPLD activity, whereas G48V does not. The decrease of lysoPLD activity of G48A and Q209L is low | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lysophospholipase D | - |
Rattus norvegicus |
| lysoPLD | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the predicted nucleotide phosphate and Mg2+ binding sites of the protein are important for lysoPLD enzymatic activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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