Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.39 extracted from

  • Koh, E.; Bandle, R.W.; Roberts, D.D.; Stracke, M.L.; Clair, T.
    Novel point mutations attenuate autotaxin activity (2009), Lipids Health Dis., 8, 4-4.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.39

Protein Variants

Protein Variants Comment Organism
H226Q the mutation impairs substrate hydrolysis, with hydrolysis of artificial substrates being disproportionately lower than that of lysophosphatidylcholine Homo sapiens
H420Q the mutation does not inhibit the wild type migration response or hydrolytic activity Homo sapiens
H434Q the mutation impairs substrate hydrolysis, with hydrolysis of artificial substrates being disproportionately lower than that of lysophosphatidylcholine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 FS-3 wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.019
-
 FS-3 mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.066
-
 lysophosphatidylcholine 16:0 wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.069
-
 lysophosphatidylcholine 18:1 mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.073
-
 lysophosphatidylcholine 18:1 wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.089
-
 lysophosphatidylcholine 18:0 wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.094
-
 lysophosphatidylcholine 16:0 mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.105
-
 lysophosphatidylcholine 18:1 mutant enzyme H434Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.115
-
 lysophosphatidylcholine 18:0 mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.12
-
 lysophosphatidylcholine 16:0 mutant enzyme H434Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.126
-
 lysophosphatidylcholine 18:1 mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.126
-
 lysophosphatidylcholine 16:0 mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.27
-
 lysophosphatidylcholine 18:0 mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.7
-
 4-nitrophenyl-TMP wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens
4.8
-
 4-nitrophenyl-TMP mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-TMP + H2O
-
 Homo sapiens ?
-
 ?
FS-3 + H2O fluorogenic lysoPLD substrate Homo sapiens 2-(6-hydroxy-3-oxo-9a,10a-dihydro-3H-xanthen-9-yl)-5-[(18-hydroxy-15-oxo-3,6,9,12-tetraoxa-16-azaoctadec-1-yl)carbamoyl]benzoic acid + (2R)-2-hydroxy-3-(phosphonooxy)propyl 6-[(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]benzoyl)amino]hexanoate
-
 ?
lysophosphatidylcholine 16:0 + H2O
-
 Homo sapiens ?
-
 ?
lysophosphatidylcholine 18:0 + H2O
-
 Homo sapiens ?
-
 ?
lysophosphatidylcholine 18:1 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ATX
-
 Homo sapiens
autotaxin
-
 Homo sapiens
NPP2
-
 Homo sapiens