Application | Comment | Organism |
---|---|---|
pharmacology | phospholipase C is considered to be one of key enzymes for the design of drug delivery system using the endocytosis route, because PLC can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | excess diacylglycerol at pH 7.5 at approximately 12% inhibits the membrane fusion activity of the enzyme | Bacillus cereus | |
o-phenanthroline | inhibits the phospholipase C hydrolytic activity of the enzyme, but not its membrane fusion activity | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-sn-diacylglycerol + phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bacillus cereus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | phospholipase C can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) at pH 4.0-7.5, highest activity at pH 5.0, 37°C. The liposomes are composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and cholesterol in a 1:1:1 molar ratio, membrane fusion is dependent of the lipid composition, overview. The enzyme takes a molten-globule state, with a large fluctuation at acidic pH, mechanism of enzyme-induced membrane fusion | Bacillus cereus | ? | - |
? | |
phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-sn-diacylglycerol + phosphocholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary and tertiary structure analysis of the enzyme at different pH values from pH 2.0-7.5, overview | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
PLC | - |
Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
additional information | secondary and tertiary structure analysis of the enzyme at different pH values from pH 2.0-7.5, overview | Bacillus cereus |