Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | 1mg/ml | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
transfected into CV-1 cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly inhibitory at concentrations of 10 mM | Rattus norvegicus | |
sodium deoxycholate | at concentrations above 1mg/ml | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
phosphatidylinositol | PLC-beta1a, 2mM free Ca2+ | Rattus norvegicus | |
0.137 | - |
phosphatidylinositol 4,5-bisphosphate | PLC-beta1a, 2mM free Ca2+ | Rattus norvegicus | |
0.369 | - |
phosphatidylinositol | PLC-beta1b, 2mM free Ca2+ | Rattus norvegicus | |
0.571 | - |
phosphatidylinositol 4,5-bisphosphate | PLC-beta1b, 2mM free Ca2+ | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | PLC-beta 1a and -beta 1b: maximum activity with 3 mM with phosphatidylinositol for substrate, PLC translocation to membranes by free Ca2+ concentration of 100 nM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
recombinant PLC -beta 1b, SDS-PAGE | Rattus norvegicus |
150000 | - |
recombinant PLC -beta 1a, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol-bisphosphate + H2O | Rattus norvegicus | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PLC-beta 1a 58fold, recombinant PLC-beta 1b 47fold, HPLC, affinity chromatography, reverse phase chromatography | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.78 | - |
PLC-beta 1b, substrate: phosphatidylinositol | Rattus norvegicus |
1.34 | - |
PLC-beta 1a, substrate: phosphatidylinositol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol + H2O | - |
Rattus norvegicus | inositol monophosphate + diacylglycerol | - |
? | |
phosphatidylinositol-bisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? |