Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diethyl ether | substrate: sphingomyelin | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | substrate: sphingomyelin | Clostridium perfringens | |
Ca2+ | substrate: sphingomyelin | Clostridium perfringens | |
Cu2+ | - |
Bacillus cereus | |
Cu2+ | substrate: sphingomyelin | Clostridium perfringens | |
dithiothreitol | - |
Bacillus cereus | |
EDTA | - |
Bacillus cereus | |
EDTA | substrate: sphingomyelin | Clostridium perfringens | |
Fe2+ | substrate: sphingomyelin | Clostridium perfringens | |
Zn2+ | substrate: sphingomyelin | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
sphingomyelin | - |
Clostridium perfringens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
extracellular | - |
Clostridium perfringens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Clostridium perfringens | |
Mg2+ | restores ability of Ca2+-inhibited PLC to hydrolyse sphingomyelin | Clostridium perfringens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | Clostridium perfringens | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | Clostridium welchii | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | Bacillus cereus 7004 | - |
1,2-diacylglycerol + phosphorylcholine | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
diethyl ether | activating | Clostridium perfringens |
mercaptoethanol | inhibitory | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus 7004 | - |
- |
- |
Clostridium perfringens | - |
strain ATCC 10543 | - |
Clostridium welchii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, column chromatography steps | Clostridium perfringens |
dialysis, column chromatography, 22fold | Bacillus cereus |
protamine precipitation, column chromatography, 23fold | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacylglycerylphosphoryl monomethylethanolamine + H2O | - |
Bacillus cereus | ? | - |
? | |
diacylglycerylphosphoryl monomethylethanolamine + H2O | - |
Bacillus cereus 7004 | ? | - |
? | |
lysophosphatidylcholine + H2O | - |
Clostridium perfringens | monoacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | - |
Clostridium welchii | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | - |
Bacillus cereus 7004 | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylethanolamine + H2O | - |
Bacillus cereus | phosphorylethanolamine + diacylglycerol | - |
? | |
phosphatidylethanolamine + H2O | - |
Clostridium perfringens | phosphorylethanolamine + diacylglycerol | - |
? | |
phosphatidylethanolamine + H2O | - |
Bacillus cereus 7004 | phosphorylethanolamine + diacylglycerol | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus | ceramide + phosphorylcholine | - |
? | |
sphingomyelin + H2O | - |
Clostridium perfringens | ceramide + phosphorylcholine | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus 7004 | ceramide + phosphorylcholine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.8 | substrate: sphingomyelin | Clostridium perfringens |