Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Myxococcus xanthus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | PdeB is strongly inhibited by 0.1 M sodium phosphate buffer (pH 6.0 and 7.0) | Myxococcus xanthus | |
Zn2+ | 28% relative activity at 0.05 mM Zn2+, using cyclic 3',5'-AMP as substrate | Myxococcus xanthus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
cyclic 2',3'-AMP | in 50 mM Tris-HCl, pH 8.0 | Myxococcus xanthus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | PdeB is stimulated about 2fold by 0.05 mM Co2+, at pH 8.0 in 50 mM Tris-HCl buffer with cyclic 3',5'-AMP as substrate | Myxococcus xanthus | |
Mn2+ | PdeB is stimulated about 2fold by 0.05 mM Mn2+, at pH 8.0 in 50 mM Tris-HCl buffer with cyclic 3',5'-AMP as substrate | Myxococcus xanthus | |
additional information | enzymatic activity is not stimulated by Ca2+, Mg2+, Fe2+, Fe3+, and Zn2+ | Myxococcus xanthus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Myxococcus xanthus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon affinity column chromatography | Myxococcus xanthus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic 2',3'-AMP + H2O | - |
Myxococcus xanthus | 3'-AMP | - |
r | |
additional information | PdeB also shows high phosphomonoesterase activity against 5'-UMP, 3'-AMP, 3'-GMP, and low activity against 5'-dAMP | Myxococcus xanthus | ? | - |
? | |
additional information | the enzyme shows no activity toward 2'-AMP and 2'-GMP, 3',5'-cGMP is either not hydrolysed or is cleaved very slowly by PdeB | Myxococcus xanthus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2',3'-cAMP phosphodiesterase | - |
Myxococcus xanthus |
PdeB | - |
Myxococcus xanthus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Myxococcus xanthus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | heat treatment at 50 or 55°C for 5 min slightly activates the phosphodiesterase activity of PdeB against 3',5'-cAMP by about 1.2fold | Myxococcus xanthus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
cyclic 2',3'-AMP | in 50 mM Tris-HCl, pH 8.0 | Myxococcus xanthus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | PdeB has slightly alkaline pH optimum (pH 7.5-8.5) for 3',5'-cAMP with 0.1 M Tris-HCl buffer | Myxococcus xanthus |