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Literature summary for 3.1.4.1 extracted from

  • Udvardy, J.; Marre, E.; Farkas, G.L.
    Purification and properties of a phosphodiesterase from Avena leaf tissues (1970), Biochim. Biophys. Acta, 206, 392-403.
    View publication on PubMed
Search for more literature for 3.1.4.1

General Stability

General Stability Organism
much enzyme activity is lost upon dialysis of the preparation against destilled water Avena sativa
repeated freezing and thawing inactivate phosphodiesterase Avena sativa

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Avena sativa
ATP
-
 Avena sativa
CN-
-
 Avena sativa
Cu2+ slight inhibition Avena sativa
CuSO4
-
 Avena sativa
EDTA
-
 Avena sativa
FAD
-
 Avena sativa
Hg2+
-
 Avena sativa
HgCl2
-
 Avena sativa
KH2PO4 slight inhibition Avena sativa
Na3AsO4 slight inhibition Avena sativa
NAD+ competitive Avena sativa
NADP+
-
 Avena sativa
NaF slight inhibition Avena sativa
phosphate slight inhibition Avena sativa
Urea slight inhibition Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 p-nitrophenylthymidine 5'-phosphate
-
 Avena sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Avena sativa
Co2+ activation Avena sativa
divalent cations activation Avena sativa
divalent cations required for activity Avena sativa
Mg2+ activation Avena sativa
Zn2+ activation Avena sativa

Organism

Organism UniProt Comment Textmining
Avena sativa
-
 oat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.183
-
-
 Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(p-nitrophenyl) phosphate + H2O
-
 Avena sativa ?
-
 ?
DNA + H2O denatured DNA. Native DNA is resistant Avena sativa DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
additional information no activity with 2',3'-cyclic nucleotides Avena sativa ?
-
 ?
additional information enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal Avena sativa ?
-
 ?
additional information broad substrate specificity Avena sativa ?
-
 ?
p-nitrophenylthymidine 5'-phosphate + H2O
-
 Avena sativa 5'-TMP + p-nitrophenol
-
 ?
poly A + H2O
-
 Avena sativa 5'-AMP
-
 ?
poly C + H2O
-
 Avena sativa 5'-CMP
-
 ?
poly U + H2O
-
 Avena sativa 5'-UMP
-
 ?
poly(I) + H2O slowly Avena sativa 5'-IMP
-
 ?
polynucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Avena sativa polynucleotides(n-1) + 5'-mononucleotides
-
 ?
RNA + H2O highly polymerized RNA: hydrolyzed at medium speed, single-stranded RNA: slowly, tRNA: not a substrate Avena sativa RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phosphodiesterase
-
 Avena sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 0.1 M Tris-acetate buffer, pH 6.8, 5 min: only 30% loss of original activity, enzyme shows considerable heat stability Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Avena sativa