Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Hordeum vulgare | |
5'-deoxyribonucleotides | end-product inhibition | Hordeum vulgare | |
5'-nucleotides | end-product inhibition | Hordeum vulgare | |
5'-ribonucleotides | end-product inhibition | Hordeum vulgare | |
Cu2+ | - |
Hordeum vulgare | |
CuSO4 | - |
Hordeum vulgare | |
EDTA | - |
Hordeum vulgare | |
glutathione | - |
Hordeum vulgare | |
KF | slight inhibition | Hordeum vulgare | |
L-cysteine | - |
Hordeum vulgare | |
Reducing agents | - |
Hordeum vulgare | |
Zn2+ | - |
Hordeum vulgare | |
ZnSO4 | slight inhibition | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
p-nitrophenylthymidine 5'-phosphate | - |
Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | slight activation | Hordeum vulgare | |
Ca2+ | slight activation | Hordeum vulgare | |
divalent cations | required for activity | Hordeum vulgare | |
K+ | slight activation | Hordeum vulgare | |
KCl | slight activation | Hordeum vulgare | |
Mg2+ | activation | Hordeum vulgare | |
MgCl2 | activation | Hordeum vulgare | |
Na+ | slight activation | Hordeum vulgare | |
NaCl | slight activation | Hordeum vulgare | |
NH4Cl | slight activation | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE | Hordeum vulgare |
100000 | - |
gel filtration and PAGE | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hordeum vulgare | the physiological function of PDE in plant cell is presumably the degradation of nucleic acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | rootlet | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.8 | - |
- |
Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | denatured DNA. Native DNA is resistant | Hordeum vulgare | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
additional information | the physiological function of PDE in plant cell is presumably the degradation of nucleic acids | Hordeum vulgare | ? | - |
? | |
oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Hordeum vulgare | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
p-nitrophenylthymidine 5'-phosphate + H2O | - |
Hordeum vulgare | 5'-TMP + p-nitrophenol | - |
? | |
polynucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Hordeum vulgare | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
RNA + H2O | - |
Hordeum vulgare | RNA(n-1) + nucleoside 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE | Hordeum vulgare |
trimer | 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
5'-PDE | - |
Hordeum vulgare |
PDE I | - |
Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Hordeum vulgare |