Literature summary for 3.1.3.B8 extracted from

Fernandes, C.; Mendes, V.; Costa, J.; Empadinhas, N.; Jorge, C.; Lamosa, P.; Santos, H.; da Costa, M.S.
Two alternative pathways for the synthesis of the rare compatible solute mannosylglucosylglycerate in Petrotoga mobilis (2010), J. Bacteriol., 192, 1624-1633.

Search for more literature for 3.1.3.B8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Petrotoga mobilis

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	addition of 20 mM EDTA leads to a residual activity of 14% in the presence of 20 mM Mn2+	Petrotoga mobilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.5	-	2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate	60°C, pH 7.0, partially purified enzyme	Petrotoga mobilis
2.6	-	2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate	60°C, pH 7.0, partially purified enzyme	Petrotoga mobilis
2.8	-	2-O-alpha-D-mannopyranosyl-3-phosphoglycerate	60°C, pH 7.0, partially purified enzyme	Petrotoga mobilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	divalent cations are not required for activity, but Mn2+, Mg2+, and Ca2+ (each 20 mM) leads to an increase in the activity by 100, 52, and 47%, respectively	Petrotoga mobilis
Mg2+	divalent cations are not required for activity, but Mn2+, Mg2+, and Ca2+ (each 20 mM) leads to an increase in the activity by 100, 52, and 47%, respectively	Petrotoga mobilis
Mn2+	divalent cations are not required for activity, but Mn2+, Mg2+, and Ca2+ (each 20 mM) leads to an increase in the activity by 100, 52, and 47%, respectively	Petrotoga mobilis
additional information	NaCl and KCl concentrations between 50 and 300 mM had no effect on the enzyme activity	Petrotoga mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O	Petrotoga mobilis	the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the rare solute mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation	2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Petrotoga mobilis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
MggB could be only partially purified, because it completely loses activity after hydrophobic interaction chromatography or size-exclusion chromatography	Petrotoga mobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.3	-	60°C, pH 7.0, partially purified enzyme, substrate: mannosyl-3-phosphoglycerate	Petrotoga mobilis
0.5	-	60°C, pH 7.0, partially purified enzyme, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate	Petrotoga mobilis
0.6	-	60°C, pH 7.0, partially purified enzyme, substrate: 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate	Petrotoga mobilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O	-	Petrotoga mobilis	?	-	?
2-O-alpha-D-mannopyranosyl-3-phosphoglycerate + H2O	-	Petrotoga mobilis	?	-	?
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O	-	Petrotoga mobilis	2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate	-	?
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O	the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the rare solute mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation	Petrotoga mobilis	2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate	-	?
additional information	UDP-glucose, ADP-glucose, GDP-mannose, ADP, GDP and UDP are dephosphorylated be the partially purified enzyme	Petrotoga mobilis	?	-	?

Synonyms

Synonyms	Comment	Organism
MggB	-	Petrotoga mobilis
MGPG phosphatase	-	Petrotoga mobilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	-	Petrotoga mobilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
55	90	55°C: about 40% of maximal activity, 90°C: about 60% of maximal activity	Petrotoga mobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	half-life: 120 h	Petrotoga mobilis
70	-	half-life: 2.8 h	Petrotoga mobilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7.5	-	Petrotoga mobilis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	pH 6.0: about 50% of maximal activity, pH 8.0: about 70% of maximal activity	Petrotoga mobilis

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Release 2023.1 (January 2023)