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Literature summary for 3.1.3.95 extracted from
- Structure of the catalytic phosphatase domain of MTMR8 implications for dimerization, membrane association and reversible oxidation (2015), Acta Crystallogr. Sect. D, 71, 1528-1539 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isoform MTMR8 phosphatase domain, hanging drop vapor diffusion method, using 10 mM N-(2-acetamido)iminodiacetic acid, 1.0 M ammonium phosphate dibasic pH 6.5 at 20°C | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A253K | the mutation enhances the kcat/Km value by 49% compared to the wild type enzyme | Homo sapiens |
| C338S | inactive | Homo sapiens |
| K255A | the mutation reduces the kcat/Km value by 66% compared to the wild type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | complete inhibition at 20 mM | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | Homo sapiens | - |
1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | Homo sapiens | - |
1-phosphatidyl-1D-myo-inositol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-agarose column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, GST-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MTMR8 | - |
Homo sapiens |
| myotubularin-related protein 8 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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