Literature summary for 3.1.3.75 extracted from

Huesa, C.; Houston, D.; Kiffer-Moreira, T.; Yadav, M.M.; Millan, J.L.; Farquharson, C.
The functional co-operativity of tissue-nonspecific alkaline phosphatase (TNAP) and PHOSPHO1 during initiation of skeletal mineralization (2015), Biochem. Biophys. Rep., 4, 196-201 .

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Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8R2H9	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
MC3T3-E1 cell	-	Mus musculus	-

General Information

General Information	Comment	Organism
physiological function	cell line MC3T3-E1 clone 14 cells express high levels of Phospho1 and low levels of tissue-nonspecific alkalinephosphatase TNAP and they mineralize their matrix strongly. Clone 24 cells express high levels of TNAP and low levels of Phospho1 and mineralize their matrix poorly. Lentiviral Phospho1 overexpression in clone 24 cells results in higher Phospho1 and TNAP protein expression and increased levels of matrix mineralization. specific inhibition of Phospho1 individually reduces mineralization levels of Phospho1 overexpressing C24 cells, whereas the simultaneous addition of inhibitors of both Phospho1 and TNAP essentially abolishes matrix mineralization	Mus musculus

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Release 2023.1 (January 2023)