Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant fragment comprising residues M19-C267 in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D123N | site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment | Homo sapiens |
D203S | site-directed mutagenesis, inactive mutant enzyme fragment | Homo sapiens |
D32N | site-directed mutagenesis, inactive mutant enzyme fragment | Homo sapiens |
D43N | site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment | Homo sapiens |
D43N/D123N | site-directed mutagenesis, inactive mutant enzyme fragment | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phosphocholine + H2O | Homo sapiens | - |
choline + phosphate | - |
? | |
O-phosphoethanolamine + H2O | Homo sapiens | - |
ethanolamine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TCT1 | PHOSPHO1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant fragments M19-C267 from Escherichia coli by nickel affinity chromatography to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-phosphoethanolamine + H2O = ethanolamine + phosphate | residues D32 and D203 of catalytic motifs are essential for catalytic activity, residues D43 and D123 are important for substrate binding and specificity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.092 | - |
purified recombinant mutant D43N enzyme fragment M19-C267, substrate O-phosphoethanolamine | Homo sapiens |
0.28 | - |
purified recombinant mutant D123N enzyme fragment M19-C267, substrate O-phosphoethanolamine | Homo sapiens |
4.96 | - |
purified recombinant wild-type enzyme fragment M19-C267, substrate O-phosphoethanolamine | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | high substrate specificity of PHOSPHO1, residual activity with beta-glycerol phosphate, 4-nitrophenyl phosphate, and ribose 5-phosphate, no activity with phospho-L-serine, diphosphate, fructose 6-phosphate, phospho-L-tyrosine, and ATP, overview | Homo sapiens | ? | - |
? | |
O-phosphocholine + H2O | - |
Homo sapiens | choline + phosphate | - |
? | |
O-phosphoethanolamine + H2O | - |
Homo sapiens | ethanolamine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure motif analysis using the amino acid sequence structural modelling and ligand docking | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | PHOSPHO1 belongs to the haloacid dehalogenase superfamily | Homo sapiens |
PHOSPHO1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
assay at | Homo sapiens |