Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.74 extracted from

  • Lee, Y.P.; Kim, D.W.; Lee, M.J.; Jeong, M.S.; Kim, S.Y.; Lee, S.H.; Jang, S.H.; Park, J.; Kang, T.C.; Won, M.H.; Cho, S.W.; Kwon, O.S.; Eum, W.S.; Choi, S.Y.
    Human brain pyridoxal-5-phosphate phosphatase (PLPP):protein transduction of PEP-1-PLPP into PC12 cells (2008), BMB Rep., 41, 408-413.
    View publication on PubMed
Search for more literature for 3.1.3.74

Application

Application Comment Organism
drug development transduction of the PEP-1-PLPP fusion protein into cells would be useful as therapeutic agent for various disorders related to this enzyme and vitamin B6 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
PLPP gene fused with a PEP-1 peptide (PEP-1-PLPP) and 6His-tag and cloned into pET-15b. Expression in Escherichia coli BL21 (DE3) transduced into PC12 cells at various times and concentrations to evaluate the transduction ability (analyzed by Western blotting, fluorescence labeled). PLP concentration in cells PC12 cells changed by transduced PEP-1-PLPP fusion protein (spectroscopic measurement) Homo sapiens

General Stability

General Stability Organism
recombinant PEP-1-PLPP fusion protein transduced into PC12 cells is stable for 36 h Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE, Western blot (rabbit anti-histidine polyclonal antibody) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxal 5'phosphate + H2O Homo sapiens
-
 pyridoxal + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96GD0
-
-

Purification (Commentary)

Purification (Comment) Organism
sonication of cells in binding buffer (5 mM imidazole, 0.5 M NaCl, 20 mM Tris-HCl, pH 7.9), centrifugation, Ni2+-nitrilotriacetic acid Sepharose affinity column, salt removed with PD10 column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 crude extract contains 0.13 units/mg, purified PEP-1-PLPP fusion protein contains 1.5 units/mg, after purification of recombinant enzyme, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring absorbance reduction at 390 nm (3 min) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxal 5'phosphate + H2O
-
 Homo sapiens pyridoxal + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
pyridoxal-5'-phosphate phosphatase PLPP Homo sapiens