General Stability | Organism |
---|---|
purified enzyme is unstable at low protein concentration, 0.002% Triton X-100 stabilizes, enzyme is unstable to freezing in the absence of glycerol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-pyridoxic acid 5'-phosphate | very effective inhibitor, 0.02 mM, 50% inhibition of pyridoxine 5-phosphate hydrolysis | Homo sapiens | |
EDTA | 0.2 mM, complete inhibition in the absence of Mg2+, 50% inhibition in the presence of 1 mM Mg2+ | Homo sapiens | |
fluoride | 2 mM, 50% inhibition | Homo sapiens | |
iodoacetate | enzyme is very sensitive to | Homo sapiens | |
molybdate | very effective inhibitor, 0.0029 mM, 50% inhibition | Homo sapiens | |
additional information | not inhibited by nucleotide phosphates, phosphoamino acids, levamisole, L-phenylalanine, L(+)-tartrate, 5 mM ATP, 5 mM phosphoglycolate, 0.5 mM alpha- or beta-glycerophosphate, 0.5 mM 3-phosphoglycerate, 0.5 mM 2,3-bisphosphoglycerate, 5 mM pyridoxine, 5 mM pyridoxamine, 5 mM 4-pyridoxic acid, 5 mM 4-pyridine-carboxaldehyde, 5 mM isonicotinate, 5 mM 3-hydroxypyridine, 5 mM salicylaldehyde, 5 mM benzaldehyde | Homo sapiens | |
N-ethylmaleimide | enzyme is very sensitive to | Homo sapiens | |
p-chloromercuribenzoate | enzyme is very sensitive to | Homo sapiens | |
p-nitrophenyl phosphate | poor, 4 mM, 50% inhibition of pyridoxine 5-phosphate hydrolysis | Homo sapiens | |
Phenyl phosphate | very poor inhibitor of pyridoxine 5-phosphate hydrolysis | Homo sapiens | |
phosphate | competitive inhibitor with respect to pyridoxine 5-phosphate, product inhibition | Homo sapiens | |
pyridoxal | weak, 11 mM, 50% inhibition of hydrolysis of pyridoxal 5-phosphate or pyridoxine 5-phosphate | Homo sapiens | |
pyridoxal 5'-phosphate | very effective inhibitor, 0.03 mM, 50% inhibition of pyridoxine 5-phosphate hydrolysis | Homo sapiens | |
pyridoxamine 5'-phosphate | 0.5 mM, 50% inhibition of pyridoxine 5-phosphate hydrolysis, less effective than pyridoxal 5-phosphate or 4-pyridoxic acid 5-phosphate | Homo sapiens | |
Zn2+ | very potent inhibitor, 50% inhibition in the presence of MgCl2 by 0.01 mM ZnCl2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00147 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
0.00255 | - |
4-pyridoxic acid 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
0.00519 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
0.034 | - |
pyridoxamine 5'-phosphate | pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | enzyme has 17% as much activity with 1 mM CaCl2 than with MgCl2 | Homo sapiens | |
Cu2+ | enzyme has 4% as much activity with 1 mM Cu2+ than with MgCl2 | Homo sapiens | |
Mg2+ | MgCl2, required for activity, best activator | Homo sapiens | |
Mn2+ | enzyme has 2% as much activity with 1 mM Mn2+ than with MgCl2 | Homo sapiens | |
additional information | not activated by 100 mM NaCl, KCl, NaBr, NaI, NaNO3 or sodium acetate | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Homo sapiens |
64000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | vitamin B6 metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
51000fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
0.575 | - |
pH 7.4, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-deoxypyridoxine 5'-phosphate + H2O | - |
Homo sapiens | 4-deoxypyridoxine + phosphate | - |
? | |
4-pyridoxic acid 5'-phosphate + H2O | the catalytic efficiency decreases in the following order: pyridoxal 5-phosphate, 4-pyridoxic acid 5-phosphate, pyridoxine 5-phosphate and pyridoxamine 5-phosphate | Homo sapiens | 4-pyridoxic acid + phosphate | - |
? | |
additional information | vitamin B6 metabolism | Homo sapiens | ? | - |
? | |
additional information | specifically dephosphorylates vitamin B6-phosphates, not: phenylphosphate, nucleotide phosphates, such as ATP, ADP, AMP, cAMP, FMN, phosphoamino acids, such as phosphoserine, phosphothreonine, phosphotyrosine, phosphoglycolate | Homo sapiens | ? | - |
? | |
p-nitrophenyl phosphate + H2O | slow hydrolysis | Homo sapiens | p-nitrophenol + phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | the catalytic efficiency decreases in the following order: pyridoxal 5-phosphate, 4-pyridoxic acid 5-phosphate, pyridoxine 5-phosphate and pyridoxamine 5-phosphate | Homo sapiens | pyridoxal + phosphate | - |
? | |
pyridoxamine 5'-phosphate + H2O | the catalytic efficiency decreases in the following order: pyridoxal 5-phosphate, 4-pyridoxic acid 5-phosphate, pyridoxine 5-phosphate and pyridoxamine 5-phosphate | Homo sapiens | pyridoxamine + phosphate | - |
? | |
pyridoxine 5'-phosphate + H2O | the catalytic efficiency decreases in the following order: pyridoxal 5-phosphate, 4-pyridoxic acid 5-phosphate, pyridoxine 5-phosphate and pyridoxamine 5-phosphate | Homo sapiens | pyridoxine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
phosphate | pH 7.4, 37°C | Homo sapiens |