Application | Comment | Organism |
---|---|---|
synthesis | transgenic expression in Pichia pastoris. Increasing isoform Alp2 copy number is detrimental to heterologous expression, clones with one copy of wild-type Alp2 produce the highest activity, clones with two, four and seven or more copies produce 70%, 25% and 10% respectively, of enzyme activity. Use of a sequence-optimized Alp2 gene increases the yield of the active enzyme by 25-50% in one/two copy clones. Reducing the temperature during heterologous expression leads to increases of 1.2-20fold suggesting that protein folding and post-translational processes may be the dominant factors limiting ALP2 expression | Lilium longiflorum |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Lilium longiflorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lilium longiflorum | Q0GYS2 | isoform Alp2 | - |
Synonyms | Comment | Organism |
---|---|---|
Alp2 | - |
Lilium longiflorum |