Cloned (Comment) | Organism |
---|---|
cloning of the cDNAs encoding the 130 kDa regulatory myosin-binding subunit MLCPa, the 38 kDa catalytic subunit MLCPc and the 20 kDa regulatory subunit MLCPsr, the full-length 130 kDa subunit is expressed in SF9 insect cells using the baculovirus expression system and sequenced | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
38000 | - |
x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
130000 | - |
x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
MLCP | - |
Gallus gallus MLCP | - |
MLCP | - |
Purification (Comment) | Organism |
---|---|
recombinant 130 kDa regulatory myosin-binding subunit, expressed in SF9 cells | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gizzard | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 130 kDa subunit MLCPa: elongated structure with 3 globular domains connected by flexible strands | Gallus gallus | ? | - |
? | |
additional information | 130 kDa subunit MLCPa: elongated structure with 3 globular domains connected by flexible strands | Gallus gallus MLCP | ? | - |
? | |
myosin light-chain phosphate + H2O | - |
Gallus gallus | myosin light-chain + phosphate | - |
? | |
myosin light-chain phosphate + H2O | - |
Gallus gallus MLCP | myosin light-chain + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |