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Literature summary for 3.1.3.46 extracted from
- 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase from frog skeletal muscle: purification, kinetics and immunological properties (1993), J. Comp. Physiol. B, 163, 89-98.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cyclic AMP dependent protein kinase | - |
Rattus norvegicus | |
| Cyclic AMP dependent protein kinase | - |
Pelophylax lessonae | |
| glycerol 1-phosphate | - |
Rattus norvegicus |  |
| glycerol 1-phosphate | counteracts fructose 6-phosphate inhibition | Pelophylax lessonae | |
| MgGTP2- | - |
Pelophylax lessonae | |
| phosphate | - |
Rattus norvegicus | |
| phosphate | counteracts inhibition by fructose-6-phosphate | Pelophylax lessonae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose 6-phosphate | - |
Pelophylax lessonae | |
| fructose 6-phosphate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
fructose 2,6-bisphosphate | - |
Pelophylax lessonae | |
| 0.0006 | - |
fructose 2,6-bisphosphate | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53300 | - |
53300, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructose 2,6-bisphosphate + H2O | Rattus norvegicus | - |
? | - |
? | |
| fructose 2,6-bisphosphate + H2O | Pelophylax lessonae | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelophylax lessonae | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 5600fold | Pelophylax lessonae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Rattus norvegicus | |
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Pelophylax lessonae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | skeletal | Rattus norvegicus | - |
| muscle | skeletal | Pelophylax lessonae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructose 2,6-bisphosphate + H2O | - |
Rattus norvegicus | fructose 6-phosphate + phosphate | - |
? | |
| fructose 2,6-bisphosphate + H2O | - |
Pelophylax lessonae | fructose 6-phosphate + phosphate | - |
? | |
| fructose 2,6-bisphosphate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| fructose 2,6-bisphosphate + H2O | - |
Pelophylax lessonae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 53300, SDS-PAGE | Rattus norvegicus |
| dimer | 53900-55800, SDS-PAGE | Pelophylax lessonae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Rattus norvegicus |
| 5.5 | - |
- |
Pelophylax lessonae |
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