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Literature summary for 3.1.3.46 extracted from

  • Myers, R.W.; Baginsky, W.F.; Gattermeir, D.J.; Geissler, W.M.; Harris, G.
    Enzymatic preparation of high-specific-activity beta-D-[6,6-3H]fructose-2,6-bisphosphate: application to a sensitive assay for fructose-2,6-bisphosphatase (2010), Anal. Biochem., 406, 97-104.
    View publication on PubMed
Search for more literature for 3.1.3.46

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli strain BL21(DE3) pLysS Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 2,6-bisphosphate + H2O Homo sapiens
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and anion exchange chromatography with triethylammonium bicarbonate as eluant Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 2,6-bisphosphate + H2O
-
 Homo sapiens D-fructose 6-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Homo sapiens
6PF2K/Fru-2,6-P2ase
-
 Homo sapiens
fructose-2,6-bisphosphatase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Homo sapiens