Protein Variants | Comment | Organism |
---|---|---|
D360A/N362A | significant decrease in catalytic activity | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0316 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
0.0738 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
0.088 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Trypanosoma brucei | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O | - |
Trypanosoma brucei | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Trypanosoma brucei | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tb927.11.6270 | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.2 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
57.5 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
140.5 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
physiological function | nuclear phosphatidylinositol 5-phosphatase (PIP5Pase) interacts with repressor activator protein RAP1 in a multiprotein complex and functions in the control of variant surface glycoprotein allelic exclusion. RAP1 binds PIP5Pase substrate phosphatidylinositol 3,4,5-trisphosphate and catalytic mutation of PIP5Pase results in simultaneous transcription of variant surface glycoproteins from all telomeric expression sites and from silent subtelomeric variant surface glycoprotein arrays. PIP5Pase and RAP1 bind to telomeric expression sites, especially at 70-bp repeats and telomeres, and their binding is altered by PIP5Pase inactivation or knockdown | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
653 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
892 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
1900 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei |