Application | Comment | Organism |
---|---|---|
molecular biology | hydrolysis of phosphatidylinositol 4,5-bisphosphate by PI(4,5)P2 5-phosphatase mediates calcium-induced inactivation of TRPV6 channels | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in HEK293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a rapamycin-inducible PI(4,5)P2 5-phosphatase is used to deplete PI(4,5)P2 in TRPV6-expressing cells. This technique is based on the translocation of the phosphatase domain of the PI(4,5)P2 5-phosphatase to the plasma membrane induced by rapamycin, which is shown to cause depletion of PI(4,5)P2. Rapamycin (100 nM) inhibits the whole-cell monovalent currents through TRPV6 channels expressing the rapamycin-inducible PI(4,5)P2 5-phosphatase constructs and has no effect in parallel controls | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Homo sapiens | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol 4,5-bisphosphate + H2O | - |
Homo sapiens | phosphatidylinositol 4-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rapamycin-inducible PI(4,5)P2 5-phosphatase | - |
Homo sapiens |