Literature summary for 3.1.3.36 extracted from

Williams, C.; Choudhury, R.; McKenzie, E.; Lowe, M.
Targeting of the type II inositol polyphosphate 5-phosphatase INPP5B to the early secretory pathway (2007), J. Cell Sci., 120, 3941-3951.

Search for more literature for 3.1.3.36

Application

Application	Comment	Organism
molecular biology	results suggest a role for INPP5B in retrograde ER-to-Golgi intermediate compartment (ERGIC) transport	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
full-length human INPP5B cDNA is cloned into pEGFP-C3 with an N-terminal FLAG tag for in vivo expression. INPP5B cDNA is cloned into pGBKT7 for yeast two-hybrid experiments and pBAC2 for expression of recombinant protein in insect cells. DNA encoding the N-terminal 229 amino acids of INPP5B is cloned into pET41EK-LIC for expression of GST-tagged protein in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C910A	mutation of the C-terminal cysteine (C910A), which abolishes prenylation of INPP5B does not affect cellular targeting of INPP5B	Homo sapiens
D524A	mutation of the aspartic acid within the conserved sequence PAWCDRIL in the 5-phosphatase domain which renders INPP5B catalytically inactive, has no effect on the targeting of INPP5B to the Golgi apparatus, ERGIC or endosomes	Homo sapiens
additional information	a deletion construct containing the N-terminus and 5-phosphatase domain (amino acids 1-564) of INPP5B is cytosolic, whereas a construct comprising the region downstream of the catalytic domain, including the RHO GAP-like domain and linker region connecting this to the catalytic domain (residues 564-913), is targeted to the Golgi to the wild-type protein. Further truncations of this C-terminal region abolishes membrane targeting, suggesting that both the linker and RHO GAP-like domains are required for correct cellular targeting	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	subcellular localisation found in Golgi apparatus and ER-to-Golgi intermediate compartment (ERGIC). INPP5B binds to specific RAB proteins within the secretory pathway, and mutational analysis indicates that RAB binding is required for efficient Golgi targeting of INPP5B	Homo sapiens	5794	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P32019	-	-

Synonyms

Synonyms	Comment	Organism
inositol polyphosphate 5-phosphatase	-	Homo sapiens
INPP5B	-	Homo sapiens

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Release 2023.1 (January 2023)