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Literature summary for 3.1.3.32 extracted from

  • Siribal, S.; Weinfeld, M.; Karimi-Busheri, F.; Mark Glover, J.N.; Bernstein, N.K.; Aceytuno, D.; Chavalitshewinkoon-Petmitr, P.
    Molecular characterization of Plasmodium falciparum putative polynucleotide kinase/phosphatase (2011), Mol. Biochem. Parasitol., 180, 1-7.
    View publication on PubMed
Search for more literature for 3.1.3.32

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain Rosetta (DE3) pLysS Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55584
-
 x * 55584, recombinant GST-tagged enzyme, mass spectrometry Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphopolynucleotide + H2O Plasmodium falciparum
-
 polynucleotide + phosphate
-
 ?
additional information Plasmodium falciparum polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. 3'-Phosphatase activity is detected, and PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3'-single-stranded overhang, but not double-stranded substrates that mimick single-strand breaks. But the recombinant PfPNKP does not reveal 5'-kinase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain Rosetta (DE3) pLysS by glutathione affinity chromatography Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphopolynucleotide + H2O
-
 Plasmodium falciparum polynucleotide + phosphate
-
 ?
additional information polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. 3'-Phosphatase activity is detected, and PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3'-single-stranded overhang, but not double-stranded substrates that mimick single-strand breaks. But the recombinant PfPNKP does not reveal 5'-kinase activity Plasmodium falciparum ?
-
 ?
additional information recombinant enzyme shows no activity with micrococcal nuclease digested DNA, nicked DNA, GC and AT rich 20-, 10-, 8-, 5-base oligonucleotides, and polyA that contain 5'-hydroxyl termini Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55584, recombinant GST-tagged enzyme, mass spectrometry Plasmodium falciparum

Synonyms

Synonyms Comment Organism
PNKP
-
 Plasmodium falciparum
polynucleotide kinase/phosphatase
-
 Plasmodium falciparum

General Information

General Information Comment Organism
evolution differences between PfPNKP and the other PNKP Walker A box/P loops: the sequence of the P-loop consensus sequence is hGxPGxGKSTh (h is hydrophobic, x is any amino acid), whereas the sequence of the P-loop of PfPNKP is IGPPGCGKTFL. Second, the difference between glutamic acid at position 330 of PfPNKP Plasmodium falciparum
physiological function PfPNKP may not be involved in single-strand break repair, since alternative terminal processing mechanisms can substitute for PfPNKP, PfPNKP DNA repair actions may be confined to overhanging termini of double-strand breaks Plasmodium falciparum