Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Pectobacterium wasabiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibits activity | Pectobacterium wasabiae | |
Co2+ | inhibits activity | Pectobacterium wasabiae | |
Cr3+ | inhibits activity | Pectobacterium wasabiae | |
Cu2+ | inhibits activity | Pectobacterium wasabiae | |
Fe3+ | inhibits activity | Pectobacterium wasabiae | |
Hg2+ | inhibits activity | Pectobacterium wasabiae | |
Ni2+ | inhibits activity | Pectobacterium wasabiae | |
Pd2+ | inhibits activity | Pectobacterium wasabiae | |
SDS | inhibits activity | Pectobacterium wasabiae | |
Zn2+ | inhibits activity | Pectobacterium wasabiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
myo-inositol hexakisphosphate | Vmax: 1714 micromol/min/mg | Pectobacterium wasabiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly enhances activity | Pectobacterium wasabiae | |
EDTA | slightly enhances activity | Pectobacterium wasabiae | |
Mg2+ | slightly enhances activity | Pectobacterium wasabiae | |
Na+ | slightly enhances activity | Pectobacterium wasabiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Pectobacterium wasabiae |
48000 | - |
predicted from cDNA | Pectobacterium wasabiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium wasabiae | A0A1D7YZ01 | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate; DSMZ 18074, the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
Purification (Comment) | Organism |
---|---|
using NH4SO4 precipitation and a HiTrap Q Sepharose XL FPLC column | Pectobacterium wasabiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1072 | - |
activity of purified appA | Pectobacterium wasabiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Pectobacterium wasabiae | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AppA | - |
Pectobacterium wasabiae |
phytase | - |
Pectobacterium wasabiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pectobacterium wasabiae |
50 | - |
- |
Pectobacterium wasabiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme retains stability when incubated at 50°C for 30 min, and over 70% activity retain. Enzyme loses activity rapidly at higher temperatures, with more than 90% activity lost after incubating at 70°C for 2 min | Pectobacterium wasabiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pectobacterium wasabiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | activity is stable over a wide range of pH, more than 80% of the total activity is retained after incubation at pH 3.0-10.0 at 37°C for 60 min | Pectobacterium wasabiae |