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Literature summary for 3.1.3.26 extracted from

  • Weaver, J.D.; Mullaney, E.J.; Lei, X.G.
    Altering the substrate specificity site of Aspergillus niger PhyB shifts the pH optimum to pH 3.2 (2007), Appl. Microbiol. Biotechnol., 76, 117-122.
    View publication on PubMed
Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment) Organism
mutants expressed in Pichia pastoris Aspergillus niger

Protein Variants

Protein Variants Comment Organism
E272K activity optimum at pH 3.2, shows improvement in substrate affinity Aspergillus niger
E272Q pH optimum similar to the wild type enzyme Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
0.13
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
0.54
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
0.77
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
4.72
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
4.79
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75850
-
 2 * 75850, gel filtration Aspergillus niger
151700
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger NRRL 3135
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger NRRL 3135 myo-inositol pentakisphosphate + phosphate
-
 ?
p-nitrophenyl phosphate + H2O little activity for p-nitrophenyl phosphate above pH 4.0 Aspergillus niger p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O little activity for p-nitrophenyl phosphate above pH 4.0 Aspergillus niger NRRL 3135 p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 75850, gel filtration Aspergillus niger

Synonyms

Synonyms Comment Organism
PhyB
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.63
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.17
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.5
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
9.3
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
17
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
23.8
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
40.67
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
88.83
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 3.5 has little activity at pH 3.0-3.5 Aspergillus niger