Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | 50% inhibitory at 100 mM | Thermotoga maritima | |
Mg2+ | slight inhibition at 100 mM | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.145 | - |
DL-myo-inositol 1-phosphate | - |
Thermotoga maritima | |
0.148 | - |
D-inositol 1-phosphate | - |
Thermotoga maritima | |
0.35 | - |
inositol-2-phosphate | - |
Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Thermotoga maritima | |
Mg2+ | - |
Thermotoga maritima | |
Mn2+ | - |
Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11400 | - |
sucrose density sedimentation | Thermotoga maritima |
28000 | - |
4 * 28000, SDS-PAGE | Thermotoga maritima |
116000 | - |
chromatography on bio-gel as well as gel electrophoresis | Thermotoga maritima |
119000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
442 | - |
- |
Thermotoga maritima |
Storage Stability | Organism |
---|---|
4°C, no loss of activity for 1 month | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-AMP + H2O | - |
Thermotoga maritima | adenosine + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Thermotoga maritima | 4-nitrophenol + phosphate | - |
? | |
D-inositol 1-phosphate + H2O | - |
Thermotoga maritima | D-inositol + phosphate | - |
? | |
DL-myo-inositol 1-phosphate + H2O | - |
Thermotoga maritima | DL-myo-inositol + phosphate | - |
? | |
myo-inositol 2-phosphate + H2O | - |
Thermotoga maritima | myo-inositol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 28000, SDS-PAGE | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
for 30 min no loss of activity | Thermotoga maritima |
100 | - |
for 10 min 20-25%, for 30 min 70% loss of activity | Thermotoga maritima |