Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ectocarpus siliculosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | complete inhibition at 1 mM | Ectocarpus siliculosus | |
Sodium fluoride | complete inhibition at 0.1 mM | Ectocarpus siliculosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
D-mannitol 1-phosphate | at pH 7.5 and 30°C | Ectocarpus siliculosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, 5 mM used in assay conditions | Ectocarpus siliculosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, calculated from amino acid sequence | Ectocarpus siliculosus |
155000 | - |
isoform M1Pase2, gel filtration | Ectocarpus siliculosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + H2O | Ectocarpus siliculosus | highly specific substrate | D-mannitol + phosphate | - |
? | |
D-mannitol 1-phosphate + H2O | Ectocarpus siliculosus CCAP 1310/4 | highly specific substrate | D-mannitol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ectocarpus siliculosus | - |
- |
- |
Ectocarpus siliculosus CCAP 1310/4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography | Ectocarpus siliculosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1-phosphate + H2O | low activity | Ectocarpus siliculosus | D-fructose + phosphate | - |
? | |
D-fructose 1-phosphate + H2O | low activity | Ectocarpus siliculosus CCAP 1310/4 | D-fructose + phosphate | - |
? | |
D-mannitol 1-phosphate + H2O | highly specific substrate | Ectocarpus siliculosus | D-mannitol + phosphate | - |
? | |
D-mannitol 1-phosphate + H2O | highly specific substrate | Ectocarpus siliculosus CCAP 1310/4 | D-mannitol + phosphate | - |
? | |
additional information | no activity with D-mannose 6-phosphate, D-fructose 6-phosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate | Ectocarpus siliculosus | ? | - |
? | |
additional information | no activity with D-mannose 6-phosphate, D-fructose 6-phosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate | Ectocarpus siliculosus CCAP 1310/4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36000, calculated from amino acid sequence | Ectocarpus siliculosus |
Synonyms | Comment | Organism |
---|---|---|
M1Pase2 | - |
Ectocarpus siliculosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
D-mannitol 1-phosphate | at pH 7.5 and 30°C | Ectocarpus siliculosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02239 | - |
D-mannitol 1-phosphate | at pH 7.5 and 30°C | Ectocarpus siliculosus |