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Literature summary for 3.1.3.21 extracted from
- Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae (2008), J. Biosci. Bioeng., 105, 508-512.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | production of 1,3-propanediol from glucose in engineered Klebsiella pneumoniae strain | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gpp2 gene from Saccharomyces cerevisiae, transformed into KG1 strain of Klebsiella pneumoniae, puc18K vector | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P40106 | GPP2; strain Y47 as donor strain for gpp2 gene | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SDS-PAGE, recombinant protein | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
changes in glucose, glycerol and 2,3-butanediol determined in engineered bacteria, new metabolic pathway of glucose via glycerol to 1,3-propanediol constructed, effect of pH on production of 1,3-propanediol, effect of dihydroxyacetone and pH, comparison of enzyme activities growing on different substrates, successful introduction of a glycerol pathway of yeast into Klebsiella pneumoniae | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol 3-phosphate + H2O | enzyme activity of GPP2 of Saccharomyces cerevisiae used for production of glycerol and 1,3-propanediol in engineered Klebsiella pneumonia | Saccharomyces cerevisiae | glycerol + phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gpp2 | - |
Saccharomyces cerevisiae |
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