Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Francisella tularensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
Thiamine monophosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg | Francisella tularensis | |
0.19 | - |
thiamine diphosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 1.38 micromol/min/mg | Francisella tularensis | |
0.47 | - |
phosphocholine | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 89.58 micromol/min/mg | Francisella tularensis | |
3.11 | - |
4-nitrophenyl phosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 11.84 micromol/min/mg | Francisella tularensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | improves activity | Francisella tularensis | |
Fe2+ | improves activity | Francisella tularensis | |
Mg2+ | improves activity | Francisella tularensis | |
Mn2+ | improves activity | Francisella tularensis | |
Zn2+ | improves activity | Francisella tularensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Francisella tularensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Francisella tularensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Francisella tularensis | 4-nitrophenol + phosphate | - |
? | |
phosphocholine + H2O | - |
Francisella tularensis | choline + phosphate | - |
? | |
thiamine diphosphate + 2 H2O | - |
Francisella tularensis | thiamine + 2 phosphate | - |
? | |
thiamine monophosphate + H2O | - |
Francisella tularensis | thiamine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcpB | - |
Francisella tularensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
thiamine diphosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 1.38 micromol/min/mg | Francisella tularensis | |
4.48 | - |
4-nitrophenyl phosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 11.84 micromol/min/mg | Francisella tularensis | |
4.85 | - |
Thiamine monophosphate | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg | Francisella tularensis | |
33.24 | - |
phosphocholine | pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 89.58 micromol/min/mg | Francisella tularensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 5.6 | - |
Francisella tularensis |