Activating Compound | Comment | Organism | Structure |
---|---|---|---|
trehalose | activates hydrolysis of glucose 6-phosphate, no activation of trehalose 6-phosphate | Phormia regina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucose | competitive inhibition of hydrolysis of glucose 6-phosphate, ineffective against hydrolysis of trehalose 6-phosphate | Phormia regina | |
phosphate | - |
Phormia regina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | 0.5 | glucose 6-phosphate | - |
Phormia regina | |
0.4 | 0.7 | trehalose 6-phosphate | - |
Phormia regina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration: 4 mM | Phormia regina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 26000 | gel filtration, sucrose density gradient centrifugation | Phormia regina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phormia regina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phormia regina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.631 | - |
trehalose activated hydrolysis of glucose 6-phosphate | Phormia regina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Deoxy-D-glucose 6-phosphate + H2O | - |
Phormia regina | 2-Deoxy-D-glucose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | - |
Phormia regina | D-fructose + phosphate | - |
? | |
glucose 6-phosphate + H2O | activated by trehalose | Phormia regina | ? | - |
? | |
trehalose-6-phosphate + H2O | - |
Phormia regina | trehalose + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
5 min, 25% loss of activity | Phormia regina |
60 | - |
5 min, 75-90% loss of activity | Phormia regina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | hydrolysis of trehalose 6-phosphate | Phormia regina |
6 | - |
hydrolysis of glucose 6-phosphate in presence of trehalose | Phormia regina |
7 | - |
hydrolysis of glucose 6-phosphate in absence of trehalose | Phormia regina |