Cloned (Comment) | Organism |
---|---|
gene FBP1, recombinant expression of the FLAG-tagged plastidic enzyme from chloroplast expression vector pWD2-CpFBP1, that includes the Chlamydomonas reinhardtii FBP1 coding region synthesized with Chlamydomonas reinhardtii chloroplast codon-bias, flanked with psbD and psbA 5' and 3' regulatory sequences, respectively, in Chlamydomonas reinhardtii strain CC125 cells. Integration of insert from expression vector pWD2-CpFBP1 into the Chlamydomonas reinhardtii chloroplast genome. Under photoautotrophic growth conditions and/or with elevated CO2 levels, this 40% elevation in FBPase activity has a negative effect overall on growth rate and biomass production | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Chlamydomonas reinhardtii | - |
D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Chlamydomonas reinhardtii CC125 | - |
D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8IKQ0 | - |
- |
Chlamydomonas reinhardtii CC125 | A8IKQ0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Chlamydomonas reinhardtii | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Chlamydomonas reinhardtii CC125 | D-fructose 6-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, recombinant FLAG-tagged enzyme CpFBP1, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
CpFBP1 | - |
Chlamydomonas reinhardtii |
CrFBPase | - |
Chlamydomonas reinhardtii |
cyanobacterial fructose-1,6-bisphosphatase | - |
Chlamydomonas reinhardtii |
cyanoFBPase-II | - |
Chlamydomonas reinhardtii |
FBP1 | gene name, nuclear gene that encodes CrFBPase | Chlamydomonas reinhardtii |
fructose 1,6-bisphosphatase | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
metabolism | Calvin cycle enzyme | Chlamydomonas reinhardtii |
metabolism | the enzyme fructose 1,6-bisphosphatase takes part in the Calvin cycle, but unlike the situation in higher plants, FBPase probably does not catalyze a rate-limiting step in the Chlamydomonas reinhardtii Calvin cycle, since under photoautotrophic growth conditions and/or with elevated CO2 levels, an elevation in FBPase activity has a negative effect overall on growth rate and biomass production | Chlamydomonas reinhardtii |
physiological function | overexpression of fructose 1,6-bisphosphatase has a detrimental effect on growth | Chlamydomonas reinhardtii |