General Stability | Organism |
---|---|
Ca2+, Sr2+ and Mg2+ maintain the structure of the enzyme in solution | Escherichia coli |
spermidine and spermine maintain the structure of the enzyme in solution | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA | Bacillus subtilis | |
Mg2+ | strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA | Escherichia coli | |
Mn2+ | strict requirement for a divalent cation, Mg2+ or Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | strict requirement for a divalent cation, Mg2+ or Mn2+ | Bacillus subtilis | |
Mn2+ | strict requirement for a divalent cation, Mg2+ or Mn2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | Bacillus subtilis | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | Escherichia coli | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | tRNA + 5'-oligoribonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change | Salmonella enterica subsp. enterica serovar Typhimurium |
ribonucleoprotein | the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change | Bacillus subtilis |
ribonucleoprotein | the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change | Escherichia coli |
ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Salmonella enterica subsp. enterica serovar Typhimurium |
ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Bacillus subtilis |
ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | Salmonella enterica subsp. enterica serovar Typhimurium | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | Bacillus subtilis | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | - |
Bacillus subtilis | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | - |
Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Escherichia coli |