Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | proteolytic processing of recombinant DNase II occurs in the lysosome, which is not involved in the activation of DNase II. The processed enzyme is relatively stable after it is formed | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
cDNA of pDNase II cloned in the pcDNA3 vector and transfected into HEK 293T cells | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | post-translational processing of the DNase II to produce the alpha1, beta and alpha2 subunits occurs in the lysosome | Sus scrofa | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
Western blot analysis, alpha subunit of DNase II | Sus scrofa |
38210 | - |
sequence analysis | Sus scrofa |
46500 | - |
Western blot analysis or calculated (addition of the mass of sugar contents to the sequence-derived mass) | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by gel filtration, 74.3fold purified | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.4 | - |
1fold purified | Sus scrofa |
327.1 | - |
74.3fold purified | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Sus scrofa | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNase II | - |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
hydrolyzes DNA under acidic conditions | Sus scrofa |